DAYA HAMBAT ENZIM TIROSINASE DARI FORMULASI SEDIAAN HAND SANITZER GEL SEBAGAI ANTIAGING BERBAHAN AKTIF TANAMAN KOMODITAS HORTIKULTURA BERUPA KOMBINASI EKSTRAK BUAH NAGA MERAH DAN BUAH LEMON

Authors

Tri Yudianto Stikes Tujuh Belas Karanganyar Jawa Tengah
Arti Wahyu Utami Stikes Tujuh Belas Karanganyar Jawa Tengah
Rifkarosita Putri Ginaris Stikes Tujuh Belas Karanganyar Jawa Tengah
Nai’matul Retno Faizah Stikes Tujuh Belas Karanganyar Jawa Tengah
Octaviana Dyah Oentari Stikes Tujuh Belas Karanganyar Jawa Tengah
Alfina Nurrahman Stikes Tujuh Belas Karanganyar Jawa Tengah
Fitria Eka Resti Wijayanti Stikes Tujuh Belas Karanganyar Jawa Tengah
Sukardi Sugeng Rahmad Stikes Tujuh Belas Karanganyar Jawa Tengah
Reni Purbanova Stikes Tujuh Belas Karanganyar Jawa Tengah

Keywords:

Antiaging, Formulation, Horticulture, Hand Sanitizer Gel

Abstract

One of the long-term losses in homeostatic balance for any organism is a sign of aging skin. The state of hyperpigmentation caused by the activity of the tyrosinase enzyme is an indicator of aging. The solution that can be recommended to prevent premature signs of aging is by caring for the skin using the most affordable pharmaceutical preparation, namely Hand Sanitizer Gel. Hand Sanitizer Gel which has anti-aging properties can be produced from bioactive compounds from horticultural commodity plants because it has been proven and is quite popular. Apart from being able to be used as an active ingredient in functional food, it can also be used as a pharmaceutical, namely the preparation of active cosmetic ingredients. This is due to the content of vitamins, antioxidants, essential oils, proteins, even phenolic compounds and other environmentally friendly active ingredients found in horticultural commodity plants, especially the fruit group used in this research. Red dragon fruit has been proven to contain organic compounds such as gallic acid, elagic acid and ferulic acid which can inhibit the melanogenesis process both in vitro and in vivo from the results of previous findings. Lemons contain saponins, flavonoids, alkaloids and tannins so they can be designed to inhibit the tyrosinase enzyme. This research aims to determine the inhibitory power of the tyrosinase enzyme from extracts of horticultural commodity plants, both red dragon fruit and lemon peels in the form of hand sanitizer gel formulations and preparations. The results of this research have proven that based on the IC50 value, horticultural commodity plant extracts from lemon peel have better inhibitory power for the tyrosinase enzyme compared to red dragon fruit, namely 3,509 µg/mL. The conclusion in this research is that horticultural commodity plants in the form of lemon peel extract are more recommended as an active substance in inhibiting the tyrosinase enzyme when compared with red dragon fruit extract based on the IC50 value even though the hand sanitizer gel dosage form has the potential to inhibit the tyrosinase enzyme.

References

Abdilah, N. A., Rezaldi, F., Pertiwi, F. D., & Fadillah, M. F. (2022). fitokimia dan skrining awal metode bioteknologi fermentasi kombucha bunga telang (Clitoria Ternatea L) sebagai bahan aktif sabun cuci tangan probiotik. MEDFARM: Jurnal Farmasi dan Kesehatan, 11(1), 44-61.

Azmi N, Hashim P, Hashim DM, Halimoon N, Nik Majid, (2014)”Anti-elastase, anti-tyrosinaseand matrix metalloproteinase-1 inhibitory activity of earthworm extracts as potential new anti-aging agent”. Asian Pac J Trop Biomed. 4(Suppl 1):S348–52.

Dolorosa, M. T., Purwaningsih, S., Anwar, E., & Hidayat, T. (2019, May). Tyrosinase inhibitory activity of Sargassum plagyophyllum and Eucheuma cottonii methanol extracts. In IOP Conference Series: Earth and Environmental Science (Vol. 278, No. 1, p. 012020). IOP Publishing.

Fadilah, F., Tedjo, A., & Heryanto, R. (2016). Penentuan Aktivitas Gabungan Ekstrak Etanol Pulosari (Alyxia reinwardtii) dan Secang (Sappan Lignum) Sebagai Inhibitor Tirosinase Yang Potensial Untuk Bahan Kosmetik Melalui Pendekatan In Silico dan In Vitro. Jurnal Jamu Indonesia, 1(1), 18-25.

Fadillah, M. F., Rezaldi, F., Safitri, E., Sasmita, H., & Somantri, U. W. (2022). Narrative review: Utilization of horticultural commodity plant tissue culture technology as a Halal biotechnology method for food and pharmaceutical purposes. International Journal Mathla’ul Anwar of Halal Issues, 2(1), 28-34.

Gazali, M., Zamani, N. P., & Batubara, I. (2014). Potensi limbah kulit buah nyirih Xylocarpus granatum sebagai inhibitor tirosinase. Depik, 3(3).

Gumilar, R., Rezaldi, F., Fadillah, M. F., Cahyono, A. T., & Yudianto, T. (2022). Antioksidan Tanaman Komoditas Hortikultura (Hias) Pada Ekstrak Etanol 96% Bunga Anggrek Bulan (Phaleonopsis amabilis L) Dari 3 Lokasi Budidaya Kultur Jaringan (In Vitro). Jurnal Kesehatan Tujuh Belas (JURKES TB), 3 (2), 326 – 322.

Junlatat, J, Fangkrathok, N & Sripanidkulchai, B. (2018). “Antioxidatif and Melanin Production Inhibitory Effects of Syzygium cumini Extracts. Songklanakarin J”. Sci. Technol. 40 (5): 1136-1143.

Mardikasari, S. A., Akib, N., & Suryani, S. (2020). Formulasi dan uji stabilitas krim asam kojat dalam pembawa vesikel etosom. Majalah Farmasi dan Farmakologi, 24(2), 49-53.

Ma’ruf, A., Safitri, E., Ningtias, R. Y., Pertiwi, F. D., & Rezaldi, F. (2022). Antibakteri Gram Positif Dan Negatif Dari Sediaan Sabun Cuci Piring Fermentasi Kombucha Bunga Telang (Clitoria ternatea L) Sebagai Produk Bioteknologi Farmasi. Jurnal Kesehatan dan Kedokteran, 1(2), 16-25.

Mustika, R., Hindun, S., & Auliasari, N. (2020). Potensi tanaman sebagai pencerah wajah alami. Jurnal Sains dan Kesehatan, 2(4), 558-562.

Ningsih, Indah Yulia, Wardhani, Lisa Kusuma, Nuryuanda, Annisa Ragdha Eka, Puspitasari, Endah, & Hidayat, Mochammad Amrun. (2022). Genistein Content and Tyrosinase Inhibitory Activity of Edamame (Glycine max) Extracts. Journal of Tropical Pharmacy and Chemistry, 6(2), 92–100.

Oentari, O. D., Cahyono, A. T., Setiawan, U., Pamungkas, B. T., & Sari, S. W. (2021). Uji Daya Hambat Pertumbuhan Jamur Patogen Dermatofita Spesies Trycopyton mentagrophytes dan Trycophyton rubrum Dari Ekstrak Etanol 96% Bunga Kecombrang. Jurnal Kesehatan Tujuh Belas (Jurkes TB), 3(1).

Oktavia, S., Novi, C., Handayani, E. E., Abdilah, N. A., Setiawan, U., & Rezaldi, F. (2021). Pelatihan Pembuatan Immunomodulatory Drink Kombucha untuk Meningkatkan Perekonomian Masa New Normal pada Masyarakat Desa Majau dan Kadudampit Kecamatan Saketi Kabupaten Pandeglang, Banten. Jurnal Pengabdian Pada Masyarakat, 6(3), 716-724.

Rezaldi, F., Taupiqurrohman, O., Fadillah, M. F., Rochmat, A., Humaedi, A., & Fadhilah, F. (2021). Identifikasi Kandidat Vaksin COVID-19 Berbasis Peptida dari Glikoprotein Spike SARS CoV-2 untuk Ras Asia secara In Silico. Jurnal Biotek Medisiana Indonesia, 10(1), 77-85.

Rezaldi, F., Ningtyas, R. Y., Anggraeni, S. D., Ma’ruf, A., Fatonah, N. S., Pertiwi, F. D., Fitriyani, F., A, L. D., US, S., Fadillah, M. F., & Subekhi, A. I. (2021). Pengaruh Metode Bioteknologi Fermentasi Kombucha Bunga Telang (Clitoria ternatea L) Sebagai Antibakteri Gram Positif Dan Negatif. Jurnal Biotek, 9(2), 169-185.

Rezaldi, F., Sasmita, H., Somantri, U. W., Fadillah, M. F., & Fathurrohim, M. F. (2021). Uji Aktivitas Antifungi Dari Ekstrak Etanol 96% Bunga

Kecombrang Yang Menginfeksi Tanaman Komoditas Hortikultura Spesies Tomat (Solanum tuberosum L). Jurnal Kesehatan Tujuh Belas (Jurkes TB), 3(1).

Sagala, Z., & Telaumbanua, K. (2020). Formulasi, uji stabilitas dan aktivitas inhibitor enzim tirosinase sediaan krim dari ekstrak buah harendong (Melastoma affine D. Don). Indonesia Natural Research Pharmaceutical Journal, 5(2), 149-173.

Yenny, R. F., Utami, A. W., Yudianto, T., Nurrahman, A., Oentari, O. D., Cahyono, A. T., Wati, D. R., Rezaldi, F., & Fadillah, M. F. (2022). Daya Ketahanan Fungi Patogen Yang Menyerang Tanaman Komoditas Hortikultura Spesies Strawberry Dari Ekstrak Etanol 96% Bunga Kecombrang. Jurnal Kesehatan Tujuh Belas (Jurkes TB), 3(2).

Roselan M , Ashari S NHF, Mohamad SMMF and R (2020) “An Improved Nanoemulsion Formulation Containing Kojic Monooleate: Optimization, Characterization and In Vitro Studies”. Molecules. 1–23.

?öhreto?lu, D., Sari, S., Barut, B., & Özel, A. (2018). Tyrosinase inhibition by some flavonoids: Inhibitory activity, mechanism by in vitro and in silico studies. Bioorganic chemistry, 81, 168-174.

Utami, A. W., Ginaris, R. P., Yudianto, T., & Wati, D. R. (2021). Uji Aktivitas Antibakteri Ekstrak Etanol 96% Bunga Kecombrang Dalam Bentuk Formulasi Dan Sediaan Masker Gel. Jurnal Kesehatan Tujuh Belas (Jurkes TB), 3(1).

Yusharyahya, S. N. (2021). Mekanisme Penuaan Kulit sebagai Dasar Pencegahan dan Pengobatan Kulit Menua: Mechanism of Skin Aging. eJournal Kedokteran Indonesia, 150-150.

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Published

2024-11-19

Issue

Vol. 4 No. 1 (2022): November 2022

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Articles

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